Gellan Gum for Plant Tissue Culture Growth Gels
Plant Tissue Culture Gellan Gum (Gellan Gum LAT) has emerged as an outstanding alternative to Agar Agar in plant tissue culture (PTC) growth gels. This application in laboratories illustrates the material’s effectiveness and adaptability in scientific settings, particularly for plant growth and development studies.
Advantages of Plant Tissue Culture Gellan Gum in PTC Growth Gels
- Strong Gel Strength: Provides a robust medium suitable for supporting plant tissue culture.
- High Transmittance: Features very high transparency (95%), allowing for clear observation of plant growth and root development.
- Brittle Gels Texture: Offers a unique texture, making it easy to handle and manipulate in lab settings.
- Heat Irreversible Gels: The gels formed by Gellan Gum LAT are heat irreversible, ensuring stability and consistency in various temperature conditions.
Avoiding Bubbles in Application
- Control of Nitrogen and Protein: To prevent bubble formation during use, it’s crucial to manage the nitrogen and protein content in the solution carefully.
Typical Dosage and Usage
- Dosage Range: The recommended dosage typically ranges from 0.015% to 0.4%, depending on the desired texture.
- Dispersion and Dissolution Methods:
- For lump-free dispersion: Premix Gellan Gum powder with other dry ingredients and pour into the liquid while stirring vigorously.
- Alternatively, disperse in a non-solvent medium like oil.
- Complete dissolution is achieved through heat treatment (boiling for 5-10 minutes or at around 85-90°C for 10-15 minutes) and can be enhanced with sequestrants.
Main Specifications
- Purity: 85%-108%
- Assay (CO2 Yielding): 3.3%-6.8%
- Total Nitrogen: Maximum 1%
- Loss on Drying: Maximum 15%
- Transmittance (0.5% Solution): Minimum 85%
- Gel Strength (0.5% Solution): Minimum 1000g/cm2
- pH (1% Solution): 5.5-7.5
- Suspension Test (0.015% Solution): Pass
Additional Information
- Ingredients: Gellan Gum (E-418), CAS: 71010-52-1, applied as a gelling agent.
- Packaging Options: Available in 1KGS/BAG, 25KGS N.W. CARTONS with inside PE bags, and 25KGS N.W. DRUMS with inside PE bags.
- Storage and Shelf Life: For optimal preservation, store in a cool, dry place with good sealing. The product has a shelf life of 24 months.
In conclusion, Gellan Gum LAT’s unique properties like high transparency, strong gel strength, and heat irreversibility make it a superior choice for plant tissue culture growth gels, offering a reliable and efficient medium for scientific research and agricultural applications.
